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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
113
Molecular Structure and Order with Second-Harmonic Generation Microscopy
characterized by a high HRS. e possible molecular origin of SHG in the muscle has been studied in
several types of preparations; the rst interesting indication came from the colocalization of the bright
SHG bands with the uorescence from GFP-myosin heavy chain in nematode muscles (Campagnola
etal., 2002). Using mouse myobrils, Plotnikov etal. (2006) showed that SHG signal does not colocal-
ize with either
α
-actinin (immunostained) or actin (labeled at the ends with rhodamine–phalloidin).
Another measurement relevant for the identication of SHG source consists
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Publisher Resources

ISBN: 9781439849156