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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
118
Second Harmonic Generation Imaging
Considering for example the two main constituents of muscle (i.e., myosin and actin), the α-helices
present in G-actin display an orientational dispersion preventing SHG, while myosin is endowed with
some extraordinarily long
α
-helices highly aligned, especially in the tail portion. Clearly, a single pro-
tein would produce too low an intensity of SHG to be detected. us, a third level of structural orga-
nization is required in which SHG-emitting proteins are arranged with a symmetry allowing further
summation of the signal up to a detectable level. ese considerations provide basis of interpreta-
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Publisher Resources

ISBN: 9781439849156