
1453D SHG Imaging and Analysis of Fibrillar Collagen Organization
a higher probability of continuing to propagate in this direction. To validate the distinction between
these tissues, t-tests were performed at 10 micron depth intervals, and the dierences were statistically
signicant (p < 0.01 in all cases).
As in the OI case, we utilized Monte Carlo simulations of these plots using the measured bulk opti-
cal parameters as inputs to decouple the initial emission directionality (i.e., F
SHG
/B
SHG
) from the SHG
propagation (based on
μ
s
and g). Representative simulations for the normal and malignant biopsies
are shown in Figures 6.11a and 6.1