
156
Second Harmonic Generation Imaging
Furthermore, by measuring SHG signal from dierent angular acceptance angles, the fraction of the
direct and scattered fractions may be separated (Han and Brown, 2010). In practice, detecting backscat-
tered signal is more suitable for strong SHG sources such as collagen; so, all reported imaging studies
of microtubules have focused on collecting the forward emission. e need to detect forward emissions
limits the utility of SHG imaging of microtubules in en bloc tissues or intact animals.
Interestingly, xatives such as paraformaldehyde abolish the SHG signal from microtubules. is
is likely because protein ...