
161Second-Harmonic Generation Imaging of Microtubules
of the zebra sh nervous system, the recording period allows imaging of nerve bers from the initial
bud stage, starting from the neural plate to the eventual formation of the neural tube. ese proof-of-
concept studies are an important step toward phenotype screening, where the nervous system develop-
ment can be compared between normal and mutant zebra sh whose genes have been manipulated
(Hsieh etal., 2008b).
A recent study has pushed the technology further by quantifying and automatically annotating the
SHG intensity from microtubules as a marker for cell division (Olivier etal., 2