
Second Harmonic Generation Imaging174
in swelling of the muscle cells, where the higher concentrations result in thicker tissues for the same
exposure time. is is due to the hyperosmotic eect of the glycerol, which replaces the lower refrac-
tive index cytoplasm. us, swelling must be taken into account when directly comparing the data
because the physical thickness aects the subsequent measured SHG signal attenuation and direction-
ality. Owing to decreased scattering and absorption losses, the 50% and 75% glycerol treatments result
in similar axial attenuation proles, suggesting that 50% glycerol already provides sucient clearing
eec ...