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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
Second Harmonic Generation Imaging174
in swelling of the muscle cells, where the higher concentrations result in thicker tissues for the same
exposure time. is is due to the hyperosmotic eect of the glycerol, which replaces the lower refrac-
tive index cytoplasm. us, swelling must be taken into account when directly comparing the data
because the physical thickness aects the subsequent measured SHG signal attenuation and direction-
ality. Owing to decreased scattering and absorption losses, the 50% and 75% glycerol treatments result
in similar axial attenuation proles, suggesting that 50% glycerol already provides sucient clearing
eec ...
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Publisher Resources

ISBN: 9781439849156