
SHG and Optical Clearing 179
However, in a separate quantitative SHG imaging with both forward- and backward-detected signals
from native murine tendon [6], we observed only decreased SHG intensity and threefold tissue swell-
ing, but no adverse eects on bril morphology in both forward and backward channels. Swelling of
the tendon over a course of 5-h clearing with 50% glycerol is shown in x–z projections and in a plot of
cross-sectional area increase with time (Figure 8.7). It must be noted that in such experiments, a sample
is restricted between a glass slide and a coverslip; so, the swelling is limited and will be dierent in vivo
depend ...