Holographic SHG Imaging 201
thanks to development of ultrafast lasers: without ultrashort pulse lasers, there would be no nonlinear
microscopy.
Nevertheless, the development of ultrafast laser sources is probably more important to holographic SHG
than it is to other nonlinear microscopy techniques. e many reasons for this can all be summarized in
one: holography is a nonscanning technique. is means that to produce an image, the light source must
illuminate the entire eld of view, as it is the case with bright-eld microscopy. erefore, to deliver the
same peak power density to the specimen that of a confocal scanning microscope, the laser source for
holography has to be much more powerful, in terms of mean power. Very high-power lasers would ...