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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
234 Second Harmonic Generation Imaging
TPF SHG
80
(a)
(b)
(c)
700
SHG
Membrane potential
1 2 3 4 5 6
Frame number
Intracellular loading Extracellular loading
7 8 9 10
650
600
550
60
40
20
0
–20
Membrane potential (mV)
Average SHG intensity (A.U.)
15
10
5
0
–5
–10
–15
–125 –100 –75–50 –25 0
Membrane potential change (mV)
25 50 75 100 125 –125–100–75 –50 –25
0
Membrane potential change (mV)
25 50 75 100 125
SHG signal change (%)
15
10
5
0
–5
–10
–15
SHG signal change (%)
–40
–60
–80
SHG
TPF
FIGURE 10.2 (See color insert.) Characterization of SHG responses to membrane potential. (a) Comparison of
two-photon uorescence (TPF) and SHG signals. TPF and SHG signals were collected simultaneously f ...
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Publisher Resources

ISBN: 9781439849156