
234 Second Harmonic Generation Imaging
TPF SHG
80
(b)
(c)
700
SHG
Membrane potential
1 2 3 4 5 6
Frame number
Intracellular loading Extracellular loading
7 8 9 10
650
600
550
60
40
20
0
–20
Membrane potential (mV)
Average SHG intensity (A.U.)
15
10
5
0
–5
–10
–15
–125 –100 –75–50 –25 0
Membrane potential change (mV)
25 50 75 100 125 –125–100–75 –50 –25
0
Membrane potential change (mV)
25 50 75 100 125
SHG signal change (%)
15
10
5
0
–5
–10
–15
SHG signal change (%)
–40
–60
–80
SHG
TPF
FIGURE 10.2 (See color insert.) Characterization of SHG responses to membrane potential. (a) Comparison of
two-photon uorescence (TPF) and SHG signals. TPF and SHG signals were collected simultaneously f ...