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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
245
11.1 Introduction
e biological molecules that exhibit second-harmonic generation (SHG) include brillar collagens,
myosin, microtubules, silk, and cellulose [1–4]. Laser scanning microscopy (LSM) allows for nonin-
vasive and nondestructive three-dimensional imaging of the SHG signal from biological samples pos-
sessing second-harmonic-generating molecules [5–9]. As an optical signal, SHG is uniquely sensitive to
the spatial organization of generating dipoles [10–14], allowing for quantitative and selective structural
characterization of second-harmonic-generating tissue. Numerous optical and structural parameters
have been derived from ...
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Publisher Resources

ISBN: 9781439849156