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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
255
Second-Harmonic Generation Imaging ofSelf-Assembled CollagenGels
is valid if the phase mismatch Δk and the index of refraction remain constant regardless of ber orienta-
tion. In fact, collagen’s birefringence of Δn ~ 0.003 (where n along the collagen monomer’s long axis > n
along the short axis) is assumed to be negligible. is seems a valid assumption since the calculation
is compared to an experimental SHG signal averaged over an ensemble of collagen bers only roughly
aligned. Furthermore, Δk may be assumed constant since the SHG interaction volume (roughly the
focal volume dimensions, ~200 nm lateral × 600 nm axial) is smaller than the mean ber diameter and
length in these cases, and can be assumed to be completely lled when c
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Publisher Resources

ISBN: 9781439849156