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Second Harmonic Generation Imaging
were distributed normally, with mean and standard deviation determined from n = 50 line-segment
measurements from SHG images of real collagen gels. Gaussian peak intensity was directly related
to the length and width, so that larger ber segments proportionally possessed more intense signal.
Furthermore, the ber edges were dened where the signal fell to 1/e
2
times the maximum intensity of
each Gaussian function. e simulated ber areas and intensities were determined so that SHG images
from cellularized gels at day 0 of culture would have similar mean intensity and signal area fraction
to