
Second Harmonic Generation Imaging276
12.4 Imaging and Characterization of Pathological Corneas
An example of the application of multiphoton microscopy in the diagnosing of corneal diseases is the
detection of infectious keratitis. Shown in Figure 12.4a is the large-area, multiphoton autouores-
cence (green) and BWSHG (blue) images of the ex vivo human cornea that have been infected with
Acanthamoeba castellinii and Pseudomonas aeruginosa. Intrinsic autouorescence shows the presence
of Acanthamoeba cysts (yellow arrowheads) and Pseudomonas bacteria (red arrowhead), and the devia-
tion of the BWSHG image pattern from its normal counter ...