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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
SHG and Multiphoton Fluorescence Imaging ofthe Eye 281
bovine corneas that have been injected with Pseudomonas aeruginosa. Images acquired at dierent
time-points (0, 6, 12, and 24 h) following injection show the eects of simulated infection on corneal
stromal collagen as a function of time. At 0 h, injected Pseudomonas aeruginosa in radiated pattern can
be observed by autouorescence imaging. At this time, the stromal collagen is largely intact with no
signs of degradation. With progression of the simulated infection process, a decrease in BWSHG signal
and increase in autouorescence are observed. In 24 h, the corneal stroma is a
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Publisher Resources

ISBN: 9781439849156