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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
289
13.1 Introduction
Multiphoton excitation microscopy provides a powerful means to study the macromolecular micro-
structure of the arterial vascular bed. e multiphoton eect restricts excited light to the focal spot,
allowing optical sectioning without the need for a confocal pinhole. is provides greatly improved
photon collection eciency (compared with single-photon confocal imaging), while maintaining three-
dimensional submicrometric spatial resolution. Moreover, owing to the reduced tissue scattering of
near-infrared excitation beams, deeper tissue penetration with minimal tissue damage can be attained.
ough second- and higher-order ...
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Publisher Resources

ISBN: 9781439849156