
Second Harmonic Generation Imaging294
Strong CARS signal is generated at the C–H and CH
2
stretch vibrational resonance,
ω
p
−
ω
s
= 2840 cm
−1
,
in lipid-rich cells and extracellular lipid droplets present in atherosclerotic plaques because of their rich
content of these bonds [29]. CARS microscopy is easily amenable to multimodal imaging via the SFG
generated between its pump and Stokes beams as demonstrated in spinal tissues [18]. Addition of a fem-
tosecond pulsed laser enables to visualize collagen with HG and elastin with MEF and therefore cover
the three major components of the arterial wall [29]. It is important to note that elastin and col ...