
Combined SHG/THG Imaging 319
14.3 Biomedical Applications
14.3.1 Imaging Contrasts of SHG and THG
In our previous ex vivo and in vivo studies, various imaging contrasts of SHG and THG microscopy
have been studied on dierent animal models and excised human tissues. In previous in vivo studies of
zebrash embryos (Chu etal. 2003; Sun etal. 2004; Chen etal. 2006) (Figures 14.6a through 14.6d), SHG
was found to arise from noncentrosymmetric structures like spindle bers (arrow in Figure 14.6a), skel-
eton muscles (arrow in Figure 14.6b), cardiac muscles (arrow in Figure 14.6c), and nerve bers (arrow in
Figure 14.6d) in the embryos, while THG ...