
Combined SHG/THG Imaging 329
14.14c), THG and SHG are found to overlap in some regions (shown white in Figure 14.14c) but do not
overlap in other regions. Since THG contrast can also be provided by the optical inhomogeneity of the
collagen bers, the SHG-overlapped THG signals can be recognized to arise from the collagen bers
(shown white in Figure 14.14c). On the other hand, the non-SHG-overlapped THG signals reect the
keratocytes lying within the collagen ber meshes (arrows in Figure 14.14c). By combining SHG with
THG modality, not only can the collagenous structures be observed, but also the cellular information in
the CS can be disti