
334 Second Harmonic Generation Imaging
melanoma cells occupying this layer are shown by THG modality with strong THG intensity (arrows
in Figure 14.17a). rough the THG signals, the larger nuclear diameter and varied cell shapes of the
supercially spreading melanoma cells can be clearly revealed as diagnostic characteristics. Owing to
a more severe invasion of the melanoma cells in the darker region, throughout the rst 100 μm layer
(Figures 14.17a through 14.17d), the regular keratinocytes are replaced by single or clustered melanoma
cells and the honeycomb architectures of the normal epidermis (Figures 14.17i and 14.17j) are almost
thoro