
Second Harmonic Generation Imaging354
15.3.2.1 3D Capability
Like other multiphoton microscopies, SHG microscopy oers 3D capability that enables the visualiza-
tion of the 3D architecture of collagen brosis. Other techniques are either restricted to 2D sections
(histological or immunochemical labeling) or show limited specicity to brillar collagen (reectance
confocal microscopy or optical coherence tomography). SHG microscopy is therefore the only tech-
nique that enables highly specic 3D imaging of intact collagen tissues.
15.3.2.2 Highly Contrasted Images/Sensitivity
e high specicity of SHG microscopy to brillar collagen results in ...