
Second Harmonic Generation Imaging364
1979, Whittaker etal. 1994). Sirius Red dye indeed aligns along the collagen molecules and enhances
the brillar collagen birefringence that is detected in polarized light microscopy. e specic advantage
of using circular light is the same as in SHG microscopy: it enables homogeneous imaging of brils with
dierent orientations in the focal plane. In practice, this technique is an excellent choice when quanti-
fying thin sections from human biopsies as a trade-o between cost and eciency. SHG microscopy is
however a better choice when imaging the 3D distribution of brosis in thick tissues and comparing ...