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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
Second Harmonic Generation Imaging364
1979, Whittaker etal. 1994). Sirius Red dye indeed aligns along the collagen molecules and enhances
the brillar collagen birefringence that is detected in polarized light microscopy. e specic advantage
of using circular light is the same as in SHG microscopy: it enables homogeneous imaging of brils with
dierent orientations in the focal plane. In practice, this technique is an excellent choice when quanti-
fying thin sections from human biopsies as a trade-o between cost and eciency. SHG microscopy is
however a better choice when imaging the 3D distribution of brosis in thick tissues and comparing ...
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Publisher Resources

ISBN: 9781439849156