
396
Second Harmonic Generation Imaging
Silver mirror
Silver mirror
Pupil lens
Collector lens
Pinhole
Objective
Objective
Animal
Tube lens
Air
Tissue
Air
Tissue
Objective
Pinhole
Band pass filter
PMT
XY scanning mirror
(b)
Excitation beam Quarter
Waveplate
Dichroic
Variable pinhole
810 nm 100 fs
FIGURE 17.2 Collagen F/B SHG measured with a single objective. Using the system illustrated in panel (a),
both the forward- and backward-directed SHG signals can be captured with a single objective, and F/B ratios can
be calculated via serial scans of the same region of interest (ROI) through dierent sized pinholes. is is pos-
sible because as illustrated in panel (b), ...