
414 Second Harmonic Generation Imaging
broblast/keratinocyte system (Chiu et al. 2005). Pena et al. have investigated the eect of collagen
accumulation around broblasts as an eect of skin aging and observed a reduced eect when using
an inhibitor agent. Figure 18.3 presents results from their investigation and shows combined SHG
(green) and two-photon uorescence (red) images of the collagen matrix and broblasts, respectively.
e images in Figure 18.3a–c were measured in a control sample without inhibitor added and an accu-
mulation of collagen around the cells can be observed. e images in Figure 18.3d–f were obtained ...