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solid-state fermented alCoholiC beverages
ey can secrets hydrolyzing enzymes to decompose macromolecular
materials and some metabolites to affect liquor flavor.
Although many studies have clearly demonstrated the broad appli-
cability of the PCR- DGGE method, the ability of DGGE to dis-
criminate among target rRNA genes is dependent on the PCR primers
used. us, the use of appropriate consensus primers has a critical
influence on the resolution of DGGE analysis in mixed microbial
systems (Fontana et al., 2005). We used two primers to fully assess
the fungal community, including yeasts and molds, and DGGE pro-
files of the 26S rRNA and 18S rRNA gene revealed different com-
positions. As assessment of the total microbial diversity by molecular
means ...