
5
Construction of Promoter and
Promoter-RBS Libraries for
Synthesis of Gene Networks
In previous chapter, some design methods have been developed to select
some well-matched biological parts to combine and produce the desired
behavior reliably. However, the above design methods need to tune some
kinetic parameters to achieve some desired steady or oscillatory state. In fact,
tuning intrinsic parameters of biological parts to fi t the designed parameter is
currently quite a diffi cult or even unfeasible task for biotechnology. Further,
it is still hard to select adequate biological parts to implement a desired
cellular function with quantitative ...