Skip to Main Content
Synthetic Gene Network
book

Synthetic Gene Network

by Bor-Sen Chen, Yu-Chao Wang
May 2014
Intermediate to advanced content levelIntermediate to advanced
216 pages
6h 47m
English
CRC Press
Content preview from Synthetic Gene Network
72 Synthetic Gene Network: Modeling, Analysis, and Robust Design Methods
5.2.1 Construction of the constitutive promoter-RBS library
Before identifying the promoter-RBS strength by measuring fl uorescence,
two important factors should be considered. First, newly synthesized
uorescent protein must undergo a series of self-modifi cations in order to
become fl uorescent (Tsien 1998). The appearance of fl uorescence reportedly
lags about 3.5 hours behind the actual synthesis of the protein (using wild-
type green fl uorescent protein (GFP) from the jellyfi sh Aequorea victoria)
(Heim et al. 1995, Albano et al. 1996). Second, the growth rate of the cell ...
Become an O’Reilly member and get unlimited access to this title plus top books and audiobooks from O’Reilly and nearly 200 top publishers, thousands of courses curated by job role, 150+ live events each month,
and much more.
Start your free trial

You might also like

Biomolecular and Bioanalytical Techniques

Biomolecular and Bioanalytical Techniques

Vasudevan Ramesh
BioCoder #4

BioCoder #4

O'Reilly Media, Inc.
BioCoder #12

BioCoder #12

O'Reilly Media, Inc.
BioCoder #11

BioCoder #11

O'Reilly Media, Inc.

Publisher Resources

ISBN: 9781466592698