
72 Synthetic Gene Network: Modeling, Analysis, and Robust Design Methods
5.2.1 Construction of the constitutive promoter-RBS library
Before identifying the promoter-RBS strength by measuring fl uorescence,
two important factors should be considered. First, newly synthesized
fl uorescent protein must undergo a series of self-modifi cations in order to
become fl uorescent (Tsien 1998). The appearance of fl uorescence reportedly
lags about 3.5 hours behind the actual synthesis of the protein (using wild-
type green fl uorescent protein (GFP) from the jellyfi sh Aequorea victoria)
(Heim et al. 1995, Albano et al. 1996). Second, the growth rate of the cell ...