
Figure 5.5. The procedure for construction of the repressor-regulated promoter-RBS library
repressor
Lib
.
the promoter-RBS strength S
u,i
. Second, according to the representative
scheme in Figure 5.2b or Figure 5.5(IV), different concentrations of inducer
can drive different fl uorescence patterns, implying that different strengths
of repressor-regulated promoter-RBS components correspond to different
concentrations of inducer. In this case, the constitutive promoter selected
from the constitutive promoter-RBS library
const
Lib
is used to produce the
stable and constitutive repressor x
r
, which is regulated by the inducer I
r
.
In a repressor-regulated ...