
82 Synthetic Gene Network: Modeling, Analysis, and Robust Design Methods
where K
a
and n
a
denote the activator-promoter binding affi nity and binding
cooperativity between the regulatory protein and activator-regulated
promoter-RBS component, respectively; S
u,i
and S
l,i
denote the maximum
and minimum activator-regulated promoter-RBS strengths from the
corresponding promoter-RBS library
activator
Lib
, respectively. In this manner,
the characteristics of activator-regulated promoter-RBS components
including the maximum and minimum promoter-RBS strengths and
binding affi nity are collected to construct the indexes of activator-regulated
promoter-RBS ...