
6
Robust Synthetic Gene
Network Designs based on
Library-search Method
After we have redefi ned the promoter and promoter-RBS libraries, we
propose some library-based search methods to effi ciently select adequate
promoters/promoter-RBSs from the corresponding libraries to engineer a
synthetic gene network with desired behaviors despite intrinsic fl uctuations
and environmental disturbances on the host cell.
6.1 H
2
Optimal Synthetic Gene Network Design: Promoter
Library-based Search Method
6.1.1 H
2
optimal synthetic gene network design and design
procedure
First, we consider a simple two-stage transcriptional cascade in Figure
6.1 (Hooshangi et al. ...