6.1.2 In silico design examples
In this section, two in silico design examples are given to illustrate the
design procedure of the proposed library-based search method. First of
all, synthetic biologists need to engineer a genetic circuit topology, and
then a dynamic model could be constructed. Finally, the proposed library-
based search method is used to obtain the most adequate promoter set
*** *
12
{, , , }
n
ccc c= …
.
6.1.2.1 Design of synthetic transcription cascade
Consider the dynamic system (6.3) of the synthetic transcriptional cascade
shown in Figure 6.1. The design specifi cations are provided and listed as
follows:
i) The desired reference trajectory to be tracked is
()
6
7
10 , 40, 80
2 10 , 40 80
r
tt
yt
t
⎧
≤>
⎪
=
⎨
×<≤
⎪
⎩
(6.8)
i.e., the refer ...