
Color Plate Section
Figure 3.1. Simulation results for synthetic transcriptional cascade network. (a) With the design
parameters in the specifi ed parameter range given in. (b) With the design parameters outside
the specifi ed parameter range.
Chapter 3
Chapter 4
tetR
λcI
lacI
TetR
GFP
gfp-aav
ColE1
kan
R
Figure 4.3. Construction of the repressilator network in the host cell, E. coli. The repressilator
is a cyclic negative-feedback loop composed of three repressor genes (the red regions) tetR, λcI,
lacI and their corresponding promoters (the gray regions) in plasmid. The compatible reporter
plasmid expresses an intermediate-stability GFP variant (gfp-aav) ...