
204 Synthetic Gene Network: Modeling, Analysis, and Robust Design Methods
Figure 7.14. The genetic transistor design example based on promoter-RBS libraries. (A) The
genetic transistor consists of repressor-regulated promoter-RBS component,
3 Tet
cLib∈
, TetR
protein coding gene and inducer, ATc. (B) The complete genetic transistor with measurement
circuit consists of three promoter-RBS components, c
1
, c
2
and c
3
, selected from promoter-
RBS libraries, Lib
const
, Lib
LacI
and Lib
TetR
, respectively. LacI protein x
LacI
represses the strength
of promoter-RBS component c
2
to decrease the concentration of TetR protein x
TetR
and the
fl uorescence g
1
of ...