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Tissue Engineering Using Ceramics and Polymers, 2nd Edition by P.X. Ma, Aldo R Boccaccini

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6

Characterisation of cells on biomaterial surfaces and tissue-engineered constructs using microscopy techniques

S.I. Anderson,    University of Nottingham School of Medicine, UK

Abstract:

Tissue engineering research can benefit enormously from the use of microscopy techniques, in particular, confocal laser scanning microscopy (CLSM). CLSM allows information on the cellular colonisation of a sample and the health and productivity of cells within and surrounding a scaffold. Many of the difficulties in processing and sectioning tissue-engineered cell-material constructs for conventional microscopy can be overcome using CLSM, sensible experimental design and appropriate sample preparation. This chapter is aimed as a practical guide to successful ...

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