
Optimization of Process Variables 53
TABLE 5 Analysis of variance (ANOVA).
Factors DOF sum of squares
(S)
variance
(V)
F -ratio
(F)
pure sum
(S')
precent
(P%)
Temperature 2 9.041 4.520 93.406 8.944 11.610
pH 2 3.859 1.929 39.873 3.672 4.884
Agitation 2 8.125 4.062 83.950 8.029 10.422
Inoculum size 2 13.562 6.781 140.118 13.465 17.479
Acetic acid 2 21.847 10.923 225.717 21.751 28.235
Succinic acid 2 20.353 10.178 210.315 20.260 26.299
Other/error 5 0.541 0.048 1.071
Total 17 77.035 100.00
FIGURE 2 Relative influence of factors and interaction.
Table 6 represents the optimum conditions required for the production of maxi-
mum 2,3-butanediol by this bacterial ...