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Conventional flux balance analysis and its applications

Abstract:

The conventional flux balance analysis method is briefly explained. Its application to the metabolic flux analysis of the Chlorella cell, under autotrophic, heterotrophic, and mixotrophic conditions, focuses on energy generation. Other applications to such single-gene knockout Escherichia coli strains, such as pflA, pta, ppc, adhE, and pykF mutants cultivated under anaerobic conditions, are also explained, as is metabolic regulation analysis based on metabolic fluxes, enzyme activities, and intracellular metabolite concentrations.

Key words

flux balance analysis

Chlorella cell

pflA mutant

pta mutant

adhE mutant

ppc mutant

pykF mutant

anaerobic conditions

photosynthetic bacteria ...

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