13C-metabolic flux analysis and its applications
Abstract:
The basic idea and the algorithm for 13C metabolic flux analysis are explained. Its application to wild-type Escherichia coli using glucose or acetate as a carbon source is explained based on isotopomer data obtained by GC-MS. Other applications are explained for metabolic flux analysis of pckA, pgi, and zwf gene knockout E. coli strains based on the isotopomer data obtained by NMR. By combining the information available from NMR, GC-MS, and metabolite balances, intracellular flux distributions in Synechocystis grown under the heterotrophic and mixotrophic conditions can be quantified, where the pentose phosphate pathway is the major pathway of glucose catabolism in the heterotrophic ...
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