Effect of a specific-gene knockout on metabolism
Abstract:
The metabolic regulation of the specific gene knockout Escherichia coli is explained in terms of 13C-metabolic fluxes, enzyme activities, intracellular metabolite concentrations, and gene expressions. It is shown that the glyoxylate pathway is activated in pckA and ppc mutants, where the cell yield can be improved as compared to the wild type. In the case of the gnd mutant E. coli, the ED pathway is activated where Mez is activated for both zwf and gnd mutants to back up the production of NADPH. The regulation mechanism of the pykF mutant E. coli is analyzed, where it is shown to be robust against such genes knockout by rerouting the pathways. Moreover, the metabolic regulations of ...
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