Nucleotide and amino acid sequences are analyzed to understand their hidden features, to discover patterns, and to determine function, structure and their evolution. The frequently used in silico analyses using molecular sequences are: sequence alignment; determining conserved regions; identification of low‐complexity region of nucleotides; gene prediction; nucleotide sequence assembling; exploring biochemical and immunogenic properties of amino acid sequences; protein structure prediction, and so on. After completing this chapter, you will learn how to use some of the sequence analytical techniques using free online software called Sequence Manipulation Suite. The original examples cited in the software suit (as “help” for explaining the programs) have been used here. In some places, the explanations may be verbatim.
6.1.1 Sequence Manipulation Suite (SMS)
Sequences are submitted to the sequence box of SMS and then analyzed according to ...