Color Inserts 271
Figure 8.8 Ethylene glycol surface density versus adsorbed serum thickness. Images
adapted from Ref. 78.
Figure 8.20 Left: contour plots of a) dry thickness of PHEMA in a MW-σ orthogonal
PHEMA gradient (scale in nm); b) dry FN thickness in a MW-σ orthogonal PHEMA/FN
gradient (scale in nm), the scales depicting the position on the substrate in parts (a) and
(b) are in cm. Right: fluorescence microscopy (10x) images of fluorescently labeled MC3T3-
E1 cells (nucleus: DAPI blue, cytoskeleton/actin: phalloidin red) cultured on PHEMA/FN
gradient substrates. Images were taken at positions on the PHEMA/FN gradient sample
marked with the numbers in part (b). The error bars associated with the thickness measure-
ments ( 0.1 nm) are smaller than the thickness increments in the contour plots. Images
adapted from Ref. 109.
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272 Color Inserts
Figure 8.22 Fluorescence photographs of BSA-adsorbed (a) bare gold and (b) linear mPEG-
2000-, (c) linear mPEG-5000-, (d) low molecular weight HPG-SH-L-, and (e) high molecular
weight HPG-SH-H grafted surfaces. Polymer films were produced by incubating the gold
surface in polymer solution at 6 g /L for 16 h. (f) Effect of the graft density on the BSA
adsorption of mPEG-, HPG-SH-L-,and HPG-SH-H-grafted surfaces. Images adapted from
Ref. 120.
Figure 8.24 Formation of a peptide-modified poly(OEGMA) brush on titanium. Images
adapted from Ref. 126.
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273
Figure 8.25 RGD functionalized PHEMA and PPEGMA brushes. Images adapted from
Ref. 128.
Figure 8.26 Fabrication of protein-functionalized polymer brushes: (i) grafting of ATRP
initiator 1 and surface-initiated ATRP; (ii) activation of hydroxyl groups with p-nitrophenyl
chloroformate (NPC); (iii) functionalization with benzylguanine derivative 2and quenching
of residual NPC groups; (iv) immobilization of AGT fusion proteins on benzylguanine-
displaying surfaces. Images adapted from ref.
129
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274 Color Inserts
D
E
Figure 9.2 (a) BSA adsorption isotherms on a piezoelectric (PZT) surface incubated at 37
C
for 1 hour and 30 minutes. The fluorescence intensity was transfer from tri-color to 256
gray scale by Adobe Photoshop 6.0. The intensity is linearly to the quantity of adsorbed
BSA, and is a function, which follows the Langmuir isotherm, of BSA concentration. Two
inserted photos are fluorescence photos in pointed experimental condition. (b) Nyquist
plot of impedance between working electrodes (PZT) and platinum counter electrode. The
voltage data (in Z measurement) is corresponding to the reference voltage. The inset is the
corresponding equivalent circuit. Reproduced with the permission from Yeh et al. (2007).
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Figure 9.7 (a) The simulation and experimental vibration spectrum of the PZT
plate/siliconmembrane. The vibration at 308, 320, 500, and 575 kHz correspond to 1.5,
2.5, 3.5 wavelength bending mode, and longitudinal mode, respectively. (b) The simulation
and experimental vibration amplitude distribution across the Si/SiO
2
membrane along the
membrane center line. The x axis is given as the distance from the center of the membrane
to the left periphery. The half length of the membrane is 1000 μm. Reproduced with the
permission from Yeh et al. (2008).
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Color Inserts 275
Figure 10.1 3D microCT image of hydroxyapatite scaffold (Engipore) loaded with sheep
BMSC and implanted in an immunodeficient mouse, showing newly formed bone (green)
onto the inner surface of the scaffold (yellow and red); the organic phase is blue. With
permission from.
4
Figure 10.3 Topography and phase image of a poly (styrene-ethylene/butylene-styrene)
(SEBS) copolymer treated by solvent annealing. (a) topography image, (b) phase image.
The phase image in (b) shows unambiguous resolution of the two different phases of the
copolymer. The phase angle contrast of two different copolymers results from the different
probe–sample interaction forces. With permission from.
24
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