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Biomedical Imaging by Sebastian Aeffner, Timo Aspelmeier, Tim Salditt

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Fig. 6.25: Propagation imaging of Deinococcus radiodurans cells [5]. (a) Hologram obtained in a single distance recording at high magnification (cone beam). (b) Phase reconstruction of single distance recording in (a), based on the CTF. (c) CTF phase reconstruction from data recorded at four source-to-object distances (defocus distances). (d) Phase reconstruction utilizing the mHIO scheme. The support information can be obtained from a holographic reconstruction or from (b). The mHIO algorithm clearly yields superior quality. Scale bars 4 μm. From [5].

6.4Grating based phase contrast

In general, the aim of phase contrast imaging is to determine ...

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