January 2026
Beginner
279 pages
10h 16m
English
Content preview from Biophysics and Molecular Biology: Tools and Techniques, 5th Edition
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4
Mass spectrometry
Mass spectrometry is an analytical technique that determines the mass of an ionic species by measuring the mass-to-charge (m/z) ratio. In mass spectrometry, an instrument called a mass spectrometer is used to determines the m/z ratio by performing four essential steps – ionization, acceleration, deflection or separation, and detection. In the process of ionization, a sample is first converted into gas-phase ions. Once the gas-phase ions are formed, the ions are accelerated so that they all have the same kinetic energy (acceleration). A magnetic field then deflects the ions according to their masses (deflection). Finally, the beam of ions passing through the machine is detected electrically (detection). A mass spectrometer ...
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