4

Mass spectrometry

Mass spectrometry is an analytical technique that determines the mass of an ionic species by measuring the mass-to-charge (m/z) ratio. In mass spectrometry, an instrument called a mass spectrometer is used to determines the m/z ratio by performing four essential steps – ionization, acceleration, deflection or separation, and detection. In the process of ionization, a sample is first converted into gas-phase ions. Once the gas-phase ions are formed, the ions are accelerated so that they all have the same kinetic energy (acceleration). A magnetic field then deflects the ions according to their masses (deflection). Finally, the beam of ions passing through the machine is detected electrically (detection). A mass spectrometer ...

Get Biophysics and Molecular Biology: Tools and Techniques by Pearson now with the O’Reilly learning platform.

O’Reilly members experience books, live events, courses curated by job role, and more from O’Reilly and nearly 200 top publishers.