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Flow cytometry
Flow cytometry is a technique used for sorting, counting, and examining cells (or microparticles) suspended in a fluid by their optical properties (light scattering and fluorescence). The term cytometry refers to the measurement of cell number and cell characteristics such as cell size, shape, cell cycle stage, DNA content, and more. It simultaneously measures and analyzes multiple physical characteristics of a single cell as they flow through a fluid stream and pass through a beam of light.
Components of a flow cytometer
A flow cytometer consists of three main systems: fluidics, optics, and electronics. The fluidic system transports the cells in a stream to the light beam for analysis. The optical system consists of excitation ...
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