Book description
The Leading Introduction to Biochemical and Bioprocess Engineering, Updated with Key Advances in Productivity, Innovation, and Safety
Bioprocess Engineering, Third Edition, is an extensive update of the world’s leading introductory textbook on biochemical and bioprocess engineering and reflects key advances in productivity, innovation, and safety.
The authors review relevant fundamentals of biochemistry, microbiology, and molecular biology, including enzymes, cell functions and growth, major metabolic pathways, alteration of cellular information, and other key topics. They then introduce evolving biological tools for manipulating cell biology more effectively and to reduce costs of bioprocesses.
This edition presents major advances in the production of biologicals; highly productive techniques for making heterologous proteins; new commercial applications for both animal and plant cell cultures; key improvements in recombinant DNA microbe engineering; techniques for more consistent authentic post-translational processing of proteins; and other advanced topics. It includes new, improved, or expanded coverage of
The role of small RNAs as regulators
Transcription, translation, regulation, and differences between prokaryotes and eukaryotes
Cell-free processes, metabolic engineering, and protein engineering
Biofuels and energy, including coordinated enzyme systems, mixed-inhibition and enzyme-activation kinetics, and two-phase enzymatic reactions
Synthetic biology
The growing role of genomics and epigenomics Population balances and the Gompetz equation for batch growth and product formation
Microreactors for scale-up/scale-down, including rapid scale-up of vaccine production
The development of single-use technology in bioprocesses
Stem cell technology and utilization
Use of microfabrication, nanobiotechnology, and 3D printing techniques
Advances in animal and plant cell biotechnology
The text makes extensive use of illustrations, examples, and problems, and contains references for further reading as well as a detailed appendix describing traditional bioprocesses.
Table of contents
- Cover Page
- About This E-Book
- Title Page
- Copyright Page
- Dedication Page
- Contents
- Preface
- About the Authors
-
Part 1: The Basics of Biology: An Engineer’s Perspective
- 1. What Is a Bioprocess Engineer?
- 2. An Overview of Biological Basics
- 3. Enzymes
-
4. How Cells Work
- 4.1. The Central Dogma
- 4.2. DNA Replication: Preserving and Propagating the Message
- 4.3. Transcription: Sending the Message
- 4.4. Translation: Going from Message to Product
- 4.5. Metabolic Regulation
- 4.6. How the Cell Senses its Extracellular Environment
- 4.7. Summary
- 4.8. Appendix: Example Regulation of Complex Pathways†
- Suggestions for Further Reading
- Problems
-
5. Major Metabolic Pathways
- 5.1. Bioenergetics
- 5.2. Glucose Metabolism: Glycolysis and the TCA Cycle
- 5.3. Respiration
- 5.4. Control Sites in Aerobic Glucose Metabolism
- 5.5. Metabolism of Nitrogenous Compounds
- 5.6. Nitrogen Fixation
- 5.7. Metabolism of Hydrocarbons
- 5.8. Biodegradation of Xenobiotics
- 5.9. Overview of Biosynthesis
- 5.10. Overview of Anaerobic Metabolism
- 5.11. Overview of Autotrophic Metabolism
- 5.12. Summary
- Suggestions for Further Reading
- Questions
- 6. How Cells Grow
-
7. Stoichiometry of Microbial Growth and Product Formation
- 7.1. Coefficients for ATP Consumption and Oxygen
- 7.2. Stoichiometric Calculations
- 7.3. Theoretical Predictions of Yield Coefficients
- 7.4. Estimation of Elemental cell Composition
- 7.5. Stoichiometry by Oxidation-Reduction Half-Reactions
- 7.6. Thermodynamics of Biological Reactions
- 7.7. Summary
- Suggestions for Further Reading
- Problems
- 8. How Cellular Information Is Altered
-
Part 2: Engineering Principles for Bioprocesses
- 9. Operating Considerations for Bioreactors for Suspension and Immobilized Cultures
-
10. Selection, Scale-Up, Operation, and Control of Bioreactors
-
10.1. Scale-Up and its Difficulties
- 10.1.1. Overview of Traditional Reactor Types
- 10.1.2. Reactors with Internal Mechanical Agitation
- 10.1.3. Bubble Column and Loop Reactor
- 10.1.4. Single-Use Bioreactors
- 10.1.5. Considerations in Aeration, Agitation, and Heat Transfer
- 10.1.6. Approaches to Scale-Up
- 10.1.7. Scale-Down and Microbioreactors
- 10.2. Bioreactor Instrumentation and Control
- 10.3. Sterilization of Process Fluids
- 10.4. Summary
- Suggestions for Further Reading
- Problems
-
10.1. Scale-Up and its Difficulties
-
11. Recovery and Purification of Products
- 11.1. Strategies to Recover and Purify Products
- 11.2. Separation of Insoluble Products
- 11.3. Cell Disruption
-
11.4. Separation of Soluble Products
- 11.4.1. Liquid–Liquid Extraction
- 11.4.2. Aqueous Two-Phase Extraction
- 11.4.3. Precipitation
- 11.4.4. Dialysis
- 11.4.5. Reverse Osmosis
- 11.4.6. Ultrafiltration and Microfiltration
- 11.4.7. Cross-Flow Ultrafiltration and Microfiltration
- 11.4.8. Adsorption
- 11.4.9. Chromatography
- 11.4.10. Electrophoresis
- 11.4.11. Electrodialysis
- 11.5. Finishing Steps for Purification
- 11.6. Integration of Reaction and Separation
- 11.7. Summary
- Suggestions for Further Reading
- Problems
-
12. Bioprocess Considerations in Using Animal Cell Cultures
- 12.1. Structure and Biochemistry of Animal Cells
- 12.2. Methods used for the Cultivation of Animal Cells
- 12.3. Bioreactor Considerations for Animal cell Culture
- 12.4. Bioreactor Systems for Animal Cell Culture
- 12.5. Products of Animal Cell Cultures
- 12.6. Summary
- Suggestions for Further Reading
- Problems
- 13. Bioprocess Considerations in Using Plant Cell Cultures
-
14. Utilizing Genetically Engineered Organisms
- 14.1. How the Product Influences Process Decisions
- 14.2. Guidelines for Choosing Host–Vector Systems
- 14.3. Process Constraints: Genetic Instability
- 14.4. Avoiding Process Problems in Plasmid Design
- 14.5. Predicting Host–Vector Interactions and Genetic Instability
- 14.6. Regulatory Constraints on Genetic Processes
- 14.7. Metabolic Engineering
- 14.8. Synthetic and Systems Biology
- 14.9. Protein Engineering
- 14.10. Summary
- Suggestions for Further Reading
- Problems
- 15. Medical Applications of Bioprocess Engineering
- 16. Bioprocesses Utilizing Mixed Cultures
- Appendix: Traditional Industrial Bioprocesses
- Index
Product information
- Title: Bioprocess Engineering: Basic Concepts, 3rd Edition
- Author(s):
- Release date: March 2017
- Publisher(s): Pearson
- ISBN: 9780132901437
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