August 2012
Intermediate to advanced
360 pages
12h 11m
English
Content preview from Biosensors for Medical Applications
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Biosensors for DNA and RNA detection and characterization
F. Davis and S.P.J. Higson, Cranfield University, UK
Abstract:
In this chapter, we describe the principles of DNA and RNA biosensing. Firstly, we discuss the structure of DNA and RNA, followed by description of the two main formats of oligonucleotide biosensors, labeled and label-free approaches. Immobilization of oligonucleotides to sensor surfaces represents a vital stage in biosensor formation. We continue by examining some of the common methods used to interrogate sensors, principally electrochemical and optical approaches. Other techniques discussed include mass sensitive methods and the use of silicon microcantilevers for detection of oligonucleotide hybridization events. ...
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