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Discovery of Novel Natural Products with Therapeutic Potential by Vincent P. Gullo

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CHAPTER 10

Screening Methodologies for the Discovery of Novel Cytotoxic Antitumor Agents

Anna M. Casazza and Byron H. Long

Publisher Summary

This chapter describes the screening methodologies for the discovery of novel cytotoxic antitumor agents. Several antitumor agents exert their cytotoxic effect because of interaction with cellular DNA, and in vitro techniques that measure this interaction can be used for antitumor drug screening. The identification of DNA binding compounds can be accomplished by observing altered migration of supercoiled, covalently closed, circular DNA in agarose gels. This approach provides several advantages for identifying DNA-binding agents. Several antitumor compounds that have proven successful in the clinic such ...

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