Cell culture systems for pancreatic research
J.D.D. Wan, S. Downes, M.J. Dunne and K.E. Cosgrove, The University of Manchester, UK
Abstract:
Two types of cell line were used to evaluate responses on electrospun polymers. The ability of Min6 (a nutrient responsive mouse insulinoma cell-line) and nes2y (a human β-cell progenitor line) cells to proliferate on an electrospun poly(ε-caprolactone) (PCL) scaffold and collagen-coated PCL scaffold was investigated. As a positive control, these cells were also grown on collagen-coated coverslips. Qualitative results were obtained taking images of the cells and scaffold using scanning electron microscopy (SEM) Quantitative results were obtained using a biochemical assay to monitor numbers of ...
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