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Essentials of Molecular Biology by Malathi V.

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9

Recombinant DNA Technology

CONCEPT OUTLINE
  • Introduction
  • DNA Isolation and Sequencing
    • DNA isolation
    • DNA sequencing Automated
    • Automated DNA sequencing
  • Tools of Recombinant DNA Technology
  • Restriction Endonucleases
    • Types of restriction endonucleases
    • Nomenclature of restriction endonuclease
    • Type II restriction endonucleases
  • Producing rDNA by Sticky-end Ligation
  • Producing rDNA by Blunt-end Ligation
    • Homopolymer tailing

       

  • Other Enzymes Used in rDNA Technology
  • Cloning Vectors
    • Types of cloning vectors
    • Cosmid vectors
    • Shuttle vectors
    • Yeast episomal plasmids
    • YAC vectors
    • Vectors for plants
    • Genes of Ti plasmid and their function
  • Nucleic Acid Hybridization and Probes
  • In Situ Hybridization
  • Molecular Cloning
    • Preparation of vector DNA
    • Preparation ...

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