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Recombinant DNA Technology
CONCEPT OUTLINE
- Introduction
- DNA Isolation and Sequencing
- DNA isolation
- DNA sequencing Automated
- Automated DNA sequencing
- Tools of Recombinant DNA Technology
- Restriction Endonucleases
- Types of restriction endonucleases
- Nomenclature of restriction endonuclease
- Type II restriction endonucleases
- Producing rDNA by Sticky-end Ligation
- Producing rDNA by Blunt-end Ligation
- Homopolymer tailing
- Homopolymer tailing
- Other Enzymes Used in rDNA Technology
- Cloning Vectors
- Types of cloning vectors
- Cosmid vectors
- Shuttle vectors
- Yeast episomal plasmids
- YAC vectors
- Vectors for plants
- Genes of Ti plasmid and their function
- Nucleic Acid Hybridization and Probes
- In Situ Hybridization
- Molecular Cloning
- Preparation of vector DNA
- Preparation ...
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