Chapter 6. Lab I-4 Assay Phosphate Concentrations in Soil Specimens

Equipment and Materials

You’ll need the following items to complete this lab session. (The standard kit for this book, available from, includes the items listed in the first group.)

Materials from Kit

  • Goggles

  • Ascorbic acid tablet, 500 mg

  • Centrifuge tubes, 15 mL

  • Centrifuge tubes, 50 mL

  • Graduated cylinder, 10 mL

  • Graduated cylinder, 100 mL

  • Molybdate reagent

  • Phosphate extraction reagent, concentrate

  • Phosphate standard, 1,000 ppm phosphate

  • Pipettes

  • Reaction plate, 24-well

  • Stirring rod

Materials you Provide

  • Gloves

  • Balance (optional)

  • Desk lamp or other light source

  • Paper (white)

  • Soft drink bottle, 500 mL (clean and empty)

  • Water, distilled or deionized

  • Specimens: soil, known ...

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