Chapter 6. Lab I-4 Assay Phosphate Concentrations in Soil Specimens

Equipment and Materials

You’ll need the following items to complete this lab session. (The standard kit for this book, available from http://www.thehomescientist.com, includes the items listed in the first group.)

Materials from Kit

  • Goggles

  • Ascorbic acid tablet, 500 mg

  • Centrifuge tubes, 15 mL

  • Centrifuge tubes, 50 mL

  • Graduated cylinder, 10 mL

  • Graduated cylinder, 100 mL

  • Molybdate reagent

  • Phosphate extraction reagent, concentrate

  • Phosphate standard, 1,000 ppm phosphate

  • Pipettes

  • Reaction plate, 24-well

  • Stirring rod

Materials you Provide

  • Gloves

  • Balance (optional)

  • Desk lamp or other light source

  • Paper (white)

  • Soft drink bottle, 500 mL (clean and empty)

  • Water, distilled or deionized

  • Specimens: soil, known ...

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