Chapter 43. Lab XI-4 DNA Analysis by Gel Electrophoresis

Equipment and Materials

You’ll need the following items to complete this lab session. (The standard kit for this book, available from, includes the items listed in the first group.)

Materials from Kit

  • Goggles

  • Agar powder

  • Beaker, 250 mL

  • Buffer, loading, 6X concentrate

  • Buffer, running, 20X concentrate

  • Cylinder, graduated, 10 mL

  • Cylinder, graduated, 100 mL

  • Leads, alligator clip (2)

  • Methylene blue stain

  • Pipettes

  • Reaction plate, 24-well

  • Ruler

Materials you Provide

  • Gloves

  • Aluminum foil

  • Balance (optional)

  • Batteries, 9V or DC power supply (see text)

  • Gel casting comb materials (see text)

  • Marking pen (Sharpie or similar)

  • Microwave oven

  • Plastic containers (see text)

  • Scissors

  • Soft drink bottle ...

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