Chapter 43. Lab XI-4 DNA Analysis by Gel Electrophoresis

Equipment and Materials

You’ll need the following items to complete this lab session. (The standard kit for this book, available from http://www.thehomescientist.com, includes the items listed in the first group.)

Materials from Kit

  • Goggles

  • Agar powder

  • Beaker, 250 mL

  • Buffer, loading, 6X concentrate

  • Buffer, running, 20X concentrate

  • Cylinder, graduated, 10 mL

  • Cylinder, graduated, 100 mL

  • Leads, alligator clip (2)

  • Methylene blue stain

  • Pipettes

  • Reaction plate, 24-well

  • Ruler

Materials you Provide

  • Gloves

  • Aluminum foil

  • Balance (optional)

  • Batteries, 9V or DC power supply (see text)

  • Gel casting comb materials (see text)

  • Marking pen (Sharpie or similar)

  • Microwave oven

  • Plastic containers (see text)

  • Scissors

  • Soft drink bottle ...

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