Integrated Microsystems

25Surface Chemistry for Cell Capture in Microfluidic Systems

ShuQi Wang

Feng Xu

Alexander Chi Fai Ip

Mrudula Somu

Xiaohu Zhao

Altug Akay

Utkan Demirci

CONTENTS

1. 25.1 Introduction
2. 25.2 Strategies to Capture Cells
   1. 25.2.1 Antibody–Ligand-Based Cell Capture
   2. 25.2.2 Aptamer-Based Cell Capture
   3. 25.2.3 DNA-Barcode-Directed Cell Capture
3. 25.3 Strategies to Improve the Efficiency of Cell Capture
   1. 25.3.1 NP Coating of Cell-Capture Surface
   2. 25.3.2 Geometry Optimization
   3. 25.3.3 Biotin–Streptavidin Affinity
4. 25.4 Applications of Cell Capture in Microfluidic Systems
   1. 25.4.1 Capture of CTCs
   2. 25.4.2 Capture of CD4⁺ T Lymphocytes
   3. 25.4.3 Stem Cells
5. 25.5 Conclusions
6. Acknowledgments
7. References

25.1 Introduction

Cell separation or sorting plays an important role ...